Histochemical characterization of neuronal NADPH-diaphorase.
نویسندگان
چکیده
منابع مشابه
Neuronal NADPH diaphorase is a nitric oxide synthase.
NADPH diaphorase histochemistry selectively labels a number of discrete populations of neurons throughout the nervous system. This simple and robust technique has been used in a great many experimental and neuropathological studies; however, the function of this enzyme has remained a matter of speculation. We, therefore, undertook to characterize this enzyme biochemically. With biochemical and ...
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The NADPH-diaphorase (NADPH-d) staining method is widely used in the investigation of both the central and peripheral nervous systems. Neuronal nitric oxide synthase (nNOS) has previously been shown to be responsible for the NADPH-d activity in neurons. However, NADPH-d activity does not always fully represent the enzyme nNOS. We investigated the distribution of NADPH-d activity and nNOS protei...
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We evaluated the neuropil distribution of the enzymes NADPH diaphorase (NADPH-d) and cytochrome oxidase (CO) in the spinal cord of the agouti, a medium-sized diurnal rodent, together with the distribution pattern and morphometrical characteristics of NADPH-d reactive neurons across different spinal segments. Neuropil labeling pattern was remarkably similar for both enzymes in coronal sections: ...
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The effects of methylmercury (MeHg) on histochemical demonstration of the NADPH-diaphorase (NADPH-d) activity in the striate cortex were studied in 4 adult cats. Two animals were used as control. The contaminated animals received 50 ml milk containing 0.42 microgram MeHg and 100 g fish containing 0.03 microgram MeHg daily for 2 months. The level of MeHg in area 17 of intoxicated animals was 3.2...
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The processing of the information coming from the external environment, including the interactions between molecular and cellular keyplayers involved in, is perhaps the “hard problem” in the cybernetic approach to the nervous system. As a whole, this information shapes the behavioral activity of an organism. The problem is faced considering the information processing flow in action from the low...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1989
ISSN: 0022-1554,1551-5044
DOI: 10.1177/37.5.2703701